Anti-cadherin-17/LI-cadherin antibody [Lic5]

Lic5     

Overview

Product name Anti-cadherin-17/LI-cadherin antibody [Lic5]
Description Mouse monoclonal antibody against cadherin-17/LI-cadherin
Tested applications Western blot (WB)
Immunohistochemistry on formalin-fixed paraffin-embedded tissue sections (IHC-P)
Species reactivity Human
Immunogen A synthetic peptide corresponding to extracellular domains 1-2 of human cadherin-17/LI-cadherin
Positive controls WB: MHCC97L hepatocellular carcinoma cell lysate
IHC-P: Human hepatocellular carcinoma tissue

Properties

Form Liquid
Storage instructions Shipping at -20 ℃. Upon delivery, aliquot and store at -20 ℃. Avoid repeated freeze/thaw cycles
Storage buffer PBS
Purity Protein G column purified tissue culture supernatant (serum-free)
Clone name Lic5
Isotype IgG2b kappa

Other information

Applications Suggested starting range
WB 0.2 to 2 µg/ml. Detect a band of approximately 120 kDa
IHC-P 0.05 to 0.5 µg/ml. Recommend to use sodium citric buffer for antigen retrieval

Prepared and Produced By

Department of Surgery
The University of Hong Kong
Technical Enquiry: nikkilee@hku.hk

For Research Use Only. Not for diagnostic or therapeutic use.



Background reference

  1. Liu LX, Lee NP, Chan VW, Xue W, Zender L, Zhang C, Mao M, Dai H, Wang XL, Xu MZ, Lee TK, Ng IO, Chen Y, Kung HF, Lowe SW, Poon RT, Wang JH, Luk JM (2009) Targeting cadherin-17 inactivates Wnt signaling and inhibits tumor growth in liver carcinoma. Hepatology 50: 1453-1463
  2. Lee NP, Poon RT, Shek FH, Ng IO, Luk JM (2010) Role of cadherin-17 in oncogenesis and potential therapeutic implications in hepatocellular carcinoma. Biochim Biophys Acta 1806: 138-145
  3. Zhu R, Wong KF, Lee NP, Lee KF, Luk JM (2010) HNF1 and CDX2 transcriptional factors bind to cadherin-17 (CDH17) gene promoter and modulate its expression in hepatocellular carcinoma. J Cell Biochem 111: 618-626
  4. Wang Y, Shek FH, Wong KF, Liu LX, Zhang XQ, Yuan Y, Khin E, Hu MY, Wang JH, Poon RT, Hong W, Lee NP, Luk JM (2013) Anti-cadherin-17 antibody modulates beta-catenin signaling and tumorigenecity of hepatocellular carcinoma. PLoS One 8: e72386

Lic5 monoclonal antibody at 1.25 µg/ml
Western blot on 20 µg MHCC97L hepatocellular carcinoma cell lysate
Lic5 monoclonal antibody at 0.06255 µg/ml
Immunohistochemical staining on paraffin embedded human hepatocellular carcinoma tissue